Fig. 7: CXCL16 promotes IL-17A secretion in epidermal Vγ2⁺ γδ T cells and CD8⁺ T cells, while its inhibition ameliorates psoriasiform pathology.

a The percentage of Vγ2⁺ γδT in γδT in Der-Ctrl and Der-Ube2l3^△Epi group. b Schematic strategy for studying the migration of Vγ2⁺ γδT cells with or without Cxcl16 for 24 hours. c The migrated cell was viewed by immunofluorence staining with DAPI (blue), five independent views of lower chambers of insects were calculated between Der-Ctrl (n = 5) and Der-Ube2l3^△Epi group (n = 5). d Next, epidermal Vγ2⁺ γδT cells were sorted and stimulated with rmCXCL16 for 48 hours, and flow cytometry to be carried out to analyze the IL-17A secretion percentage. e Percentage of Vγ2⁺ IL-17A⁺T in CD3⁺T in isotype and rmCXCL16 group. n = 3 per group. f, g Ube2l3^△Epi mice were treated with isotype antibodies or anti-CXCL16 mAb. H&E staining of back skin and ear skin was performed. h Epidermal single cells were stimulated with or without recombinant human CXCL16 and flow cytometric analysis of IL-17A was showed. i The percentage of IL-17A⁺ CD3⁺T /CD3⁺Tcells, CD8⁺IL-17A⁺/IL-17A⁺ T cells, CD4⁺IL-17A⁺/IL-17A⁺T cells in isotype cytokine and rhCXCL16 cytokine. j In wild-type (WT) mice, intradermal injection of recombinant mouse CXCL16 (rmCXCL16) and rmIL-23 every other day for 14 days. k H&E staining of and ear skin was performed. l Skin thickness measurements (using vernier calipers) of ear skin in control ear, rmCXCL16 ear, n = 5 per group, and control ear, rmIL-23 group, n = 3 per group. m–o Flow cytometry analysis of Vγ2⁺ γδT cell and Vγ3⁺ γδT cells in γδT, IL-17A in CD3⁺T cells, and CXCR6⁺IL-17A⁺ in CD3⁺T cells. n = 4 per group. Data are presented as mean ± SEM, and p-values were calculated by unpaired, two-tail student’s t-test (a, c, e, g, l), paired, two-tail student’s t-test (i, k, m, n, o). The Figure d, f, h, j was created in BioRender. Chen, X. (2025) https://BioRender.com/a73juv2.