Fig. 5: Quantification of cytosolic delivery of extracellular mitochondria using NLuc complementation.
From: Quantitative cellular characterization of extracellular mitochondria uptake and delivery
A, B SEC fractionation profiles of conditioned media A or isolated mitochondria B derived from cells expressing HiBiT-OMP25. NLuc activity was measured in fractions 1–18 following complementation with recombinant LgBiT and is shown in blue. C Quantification of cytosolic delivery of HiBiT-OMP25 from fractions F4, F4′, or F16 into acceptor HeLa cells expressing LgBiT. Three independent experiments were performed, each including three technical replicates. Each dot represents a replicate. Error bars indicate standard deviation, center represents the means. *Statistical significance was assessed by unpaired, two-tailed t-test comparing F4 or F4′ to F16; p < 0.01.
