Fig. 1: Outline of CnAcs1 inhibitor screen and validation strategy leading to the identification of isoxazole 1.
From: Discovery and mechanism of a highly selective, antifungal acetyl-CoA synthetase inhibitor
A The number of compounds evaluated in the primary screen, hit validation, and counter screening steps of the screening campaign are shown. B The structure of the hit isoxazole 1 is shown with the functionally distinct regions of the molecule highlighted. C The IC50 of isoxazole 1 towards CnAcs1 is indicated; the curve is representative of two independent experiments. The mean IC50 calculated from the two independent replicates performed in technical triplicate is shown with the standard deviation. R2 value for goodness-of-fit for the two experiments used to compute the IC50 were 0.996 and 0.995. Source data are provided as a Source data file.
