Fig. 3: Deletion mutant of SGF29 is resistant to isoxazole 1.
From: Discovery and mechanism of a highly selective, antifungal acetyl-CoA synthetase inhibitor
A Schematic of resistant mutant identification through iterative passaging into higher concentrations of isoxazole 1 using three parental strains: H99, the acl1∆ mutant, and the kbc1∆ mutant. B Diagram showing domains of the Sgf29 protein and the truncation mutant isolated from the passaging experiment. The green color indicates the Tudor domain of SGF29. The cross-hatches denote the regions of the proteins that are not translated in the truncation mutants. C Competitive growth assay between mNEON-tagged H99 and the sgf29∆ mutant at the indicated concentrations of isoxazole 1. The ratio of the two strains was determined by flow cytometry with data represented as mean and standard deviation of three independent replicates. The difference between the sgf29∆ mutant and H99 was statistically significant at 16 µg/mL (p = 0.0465 two-sided, unpaired Student’s t-test). Source data are provided as a Source data file.
