Fig. 1: Elevated CCL20 levels promote PF development. | Nature Communications

Fig. 1: Elevated CCL20 levels promote PF development.

From: The CCL20–integrin α5β1 interaction enhances TGF-β/Smad signaling to promote fibroblast activation in pulmonary fibrosis

Fig. 1

a Heatmap showing the chemokine contents in BALF from PBS- or BLM-challenged mice detected with a cytokine array. b CCL20 concentrations in the BALF of mice subjected to multiple BLM exposures (n = 5 samples for Vehicle, 1BLM, 4BLM, 5BLM; n = 7 samples for 2BLM; n = 3 samples for 3BLM; n = 6 samples for 6BLM). c Schematic overview of the experimental design for (d–g). Masson’s trichrome staining (d), Crs (e) (n = 6 samples for PBS + Vehicle, PBS + BLM, CCL20 + BLM; n = 5 samples for CCL20 + Vehicle), hydroxyproline content (f) (n  =  6 samples for PBS + Vehicle, CCL20 + Vehicle, CCL20 + BLM; n = 7 samples for PBS + BLM), and the expression levels of Col1a1, Col3a1, Fibronectin and Acta2 in the lung tissues (g) (n = 5 samples per group) of PBS- or BLM-challenged mice treated with or without CCL20. Scale bar, 200 μm (d). h Schematic overview of the experimental design for (i, j). Masson’s trichrome staining (i) and Crs (j) of PBS- or BLM-challenged mice treated with or without the CCL20 Ab (n = 6 samples per group). Scale bar, 1 mm (i). k Hydroxyproline content in PBS- or BLM-challenged mice treated with or without the CCL20 Ab (n = 6 samples per group). The data are presented as the means ± SEM. The data in (b) were analyzed by two-tailed Student’s t-test. The data in e, f, j, k were analyzed by one-way ANOVA. *p < 0.05; **p < 0.01; ***p < 0.001. Source data are provided as a Source data file.

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