Fig. 1: Inactivation of IL→NAcSh neurons impairs social memory retrieval.
From: Hippocampal-cortical interactions in the consolidation of social memory
a Social familiarization/recognition task. A subject mouse interacted with a novel conspecific three times (defined as FN, FN’, then FN”) for 5 min each with 5-min intervals. Next day, the subject mouse interacted with a familiarized (F), a novel (N), and a littermate (L) conspecific. b Timeline for the optogenetic and chemogenetic manipulation of IL→NAcSh neurons. c Left: Schematic for optogenetic manipulation. Right: mPFC (top) and NAcSh (bottom). Scale: 1000 μm and 500 μm. d Optogenetic inactivation during familiarization. Left: Both groups showed significantly reduced interaction times. Two-way ANOVA with repeated measures (RM): YFP/NpHR, n = 13/13 mice; F2,48 = 0.1333, p = 0.8755; Right: Both groups interacted longer with novel conspecifics. F2,48 = 3.509, *p = 0.0378. e Left: Schematic for chemogenetic manipulation. Right: IL (top) and NAcSh (bottom). Scale: 1000 μm and 500 μm. f Chemogenetic inactivation during offline. Left: Both groups showed reduced interactions during familiarization. Two-way RM ANOVA: mCherry/hM4Di, n = 14/12 mice; F2, 48 = 2.593, p = 0.0852. Right: Both groups interacted longer with novel conspecifics. F2, 48 = 0.6990, p = 0.5021. g Optogenetic inactivation during recognition. Left: Both groups showed reduced interactions during familiarization. Two-way RM ANOVA: YFP/NpHR, n = 13/13 mice; F2,48 = 0.3249, p = 0.7242. Right: NpHR mice showed similar interaction times with different conspecifics. F2,34 = 4648, p = 0.4648. Data presented as mean ± SEM; each dot represents one mouse. Statistics used two-way RM ANOVA test with two-sided Tukey-corrected post hoc comparisons. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a source data file. The mouse illustration in (a) and the schematic drawings of the brain in (c) and (e) are reprinted from Cell Reports, Volume 35, Issue 6, Park, et al., Social isolation impairs the prefrontal-nucleus accumbens circuit subserving social recognition in mice, 109104, Copyright (2021), with permission from Elsevier.
