Fig. 6: Offline vCA1 inactivation disrupts social memory representations in IL→NAcSh neurons.
From: Hippocampal-cortical interactions in the consolidation of social memory
a Left: Schematic for the expression of GCaMP6f in IL→NAcSh and hM4Di in vCA1→IL neurons. Right: GCaMP6f in IL (top, scale: 500, 100 µm); hM4Di in vCA1 (bottom, scale: 1000 µm). b Extended social familiarization/recognition task with offline vCA1→IL inactivation. Mice met two novel mice (FN1 and FN2) during familiarization; interacted with familiar (F1, F2), novel (N1, N2), and littermate (L1, L2) conspecifics during recognition. c Left: mCherry mice showed reduced interaction time during familiarization. One-way RM ANOVA: F2.800,19.60 = 9.807, ***p = 0.0004. Right: mCherry mice showed longer interaction with novel mice during recognition. F5,35 = 21.59, ****p < 0.0001. d Left: hM4Di mice showed reduced interaction times during familiarization. One-way RM ANOVA: F2.929,23.43 = 17.23, ****p < 0.0001. Right: hM4Di mice showed comparable interaction with familiar and novel conspecifics, but less interaction with littermates during recognition. F3.259,26.07 = 10.61, ****p < 0.0001. e mCherry mice exhibited increased overlapping social cells after familiarization, whereas hM4Di mice did not. Two-way RM ANOVA: F2, 30 = 3.384, *p = 0.0473. f Familiarity CCGP (familiar vs. novel) decoding. g Geometry from a mCherry mouse with high familiarity CCGP performance. h Geometry from a hM4Di mouse with chance-level performance. i High familiarity CCGP performance in mCherry, but not hM4Di mice. Two-way RM ANOVA: F1,15 = 9.343, **p = 0.0080. j Top: Familiarity decoding (familiar vs. FN). Bottom: High CCGP performance in mCherry only. Two-way RM ANOVA: F1, 15 = 6.444, *p = 0.0227. k Top: Familiarity decoding (littermate vs. FN). Bottom: Both groups showed high decoding performance. Two-way RM ANOVA: F1, 15 = 4.013, p = 0.0636. l Top: Familiarity decoding (littermate vs. novel). Bottom: Both groups showed high decoding performance. Two-way RM ANOVA: F1, 15 = 1.550, p = 0.2322. m Top: Cross-familiarity CCGP (familiar/littermate vs. novel). Bottom: High performance in mCherry only. Two-way RM ANOVA: F1, 15 = 4.858, *p = 0.0436. mCherry/hM4Di, n = 8/9 mice. Data presented as mean ± SEM; each dot represents one mouse. One- or two-way RM ANOVA with two-sided Sidak or Tukey-corrected post hoc comparisons. p values presented in figures. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a source data file. The schematic diagram of the brain in (a) and the mouse illustration in (b) are reprinted from Cell Reports, Volume 35, Issue 6, Park, et al., Social isolation impairs the prefrontal-nucleus accumbens circuit subserving social recognition in mice, 109104, Copyright (2021), with permission from Elsevier.
