Fig. 2: Identification of the causal gene for N-dependent flowering regulation. | Nature Communications

Fig. 2: Identification of the causal gene for N-dependent flowering regulation.

From: Photoperiodic flowering regulators are required for nitrogen-dependent flowering delay in rice under long-day condition

Fig. 2

a, b Exon-intron structure of Hd with polymorphisms inducing amino acid exchanges in our population. c Box plots showing days to heading of 149 Japanese cultivars in three N levels between eight functional and non-functional alleles combinations in Hd6, Hd1 and Hd2. The data corresponds to that shown in Fig. 1a. Haplotype information of each cultivar is shown in Supplementary Data 1. +/+, functional haplotype. −/−, non-functional haplotype. Letters indicate significant differences in each pattern of haplotype (P < 0.05, Tukey’s HSD test). The Tukey–Kramer’s HSD tests were separately conducted among the haplotype combinations. N means the number of cultivars having the haplotype pattern. d NIL-Hd1, NIL-Hd2 and NIL-Hd6 were introgressed with Kasalath segment carrying Hd1 (−/−), Hd2 (−/−) and Hd6 (+/+), respectively. White and red segments indicate Nipponbare and Kasalath with the names of the selection markers, respectively. e Boxplot for comparison of DTH of the NILs and Nipponbare (hd6/Hd1/Hd2) under normal and high N condition. P-values were calculated by two-tailed paired t-tests. The DTH date were obtained from more than four biological replicates. The box plot boundaries reflect the interquartile range; the centre line is the median and the whiskers represent 1.5× the interquartile range from the lower and upper quartiles. Source data are provided as a Source Data file.

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