Extended Data Fig. 2: TuA and TuB positively regulate plant immunity.
a, b, VIGS of both NbTuA and NbTuB in N. benthamiana significantly increased P. infestans lesion sizes compared to TRV2:GFP control (n represents the total number of measured lesions with 3 biological replicates, **** P < 0.0001, unpaired t test). Left leaves in (b) show the phenotype of VIGS plants. Right leaves are P. infestans infected leaf images under UV light. c–f, Graph shows a significant decrease in P. infestans lesion and sporangia after inoculation with HB0914-2 on StTuA-GFP and StTuB-GFP transgenic potato lines compared to the Desiree control (for sporangia counting, n = 9). g–j, Transgenic StTuA-GFP and StTuB-GFP N. benthamiana lines showed significantly increased late blight resistance. k, l, Transgenic N. benthamiana lines overexpressing StTuA-GFP and StTuB-GFP showed a significant decrease in P. capsici lesion size compared to the N. benthamiana (WT) control. In c-f, g, i, k, data are presented as mean values +/− SEM with different asterisks refer to significant difference based on one-way ANOVA, comparing the mean of each column with the mean of the control N. b (n represents the total number of measured lesions with 3 biological replicates, ** P < 0.01, *** P < 0.001, **** P < 0.0001). The white circle represents the P. capsici lesion area. h, j, l, representative leaf images taken under UV light. In a, c, e, g, I and k, centre lines show the medians; box limits indicate the 25th and 75th percentiles; whiskers extend to 1.5 × the interquartile range from the 25th and 75th percentiles, the top line represents maxima and bottom line represents minima, each point represents the lesion area of an individual leaf with precise n numbers in figures. Exact P values are shown in source data with 95% confidence intervals.
