Extended Data Fig. 9: Interaction of SlPR1 with the WT and mutant FolSvp2 proteins.
From: Plant PR1 rescues condensation of the plastid iron-sulfur protein by a fungal effector
a, Y2H assays showing the interaction of SlPR1 with the WT and mutant FolSvp2 proteins. b, Split-LUC assays (left) showing the interaction of SlPR1 with the WT and mutant FolSvp2 proteins without the native signal peptide. Scale bars = 1 cm. Western blot analysis (right) was performed to show the expression of the indicated proteins. Proteins extracted were probed with α-LUC and α-actin. c, Co-IP assays showing the interaction of SlPR1 with the WT and mutant SlPR1SPFolSvp2 proteins. The indicated constructs were transiently expressed in N. benthamiana leaves for 3 days, and the proteins copurified with GFP-Trap beads were probed with α-GFP and α-RFP. The input proteins were subjected to Western blotting with α-GFP, α-RFP and α-actin. All the experiments were repeated three times with similar observations.
