Extended Data Fig. 2: Identification of the ubiquitinated sites in FolSvp2 expressed in Fol and in planta. | Nature Plants

Extended Data Fig. 2: Identification of the ubiquitinated sites in FolSvp2 expressed in Fol and in planta.

From: Plant PR1 rescues condensation of the plastid iron-sulfur protein by a fungal effector

Extended Data Fig. 2

a, LC-MS/MS identification of FolSvp2 ubiquitination in Fol. After MG132 treatment for 2 hours, the immunoprecipitated FolSvp2R-GFP was subjected to LC‒MS/MS analysis. The coverage (as highlighted in bold) of the FolSvp2 protein sequence was 75.9%. The ubiquitinated residues identified are highlighted in blue. b, Spectra showing FolSvp2 ubiquitination at K107 and K215. The representative y21 and y10 ions represent the assignment of K107 and K215 ubiquitination, respectively. c, LC-MS/MS identification of FolSvp2 ubiquitination in N. benthamiana. After MG132 treatment for 4 hours, the immunoprecipitated FolSvp2-GFP was subjected to LC‒MS/MS analysis. The coverage (as highlighted in bold) of the FolSvp2 protein sequence was 82.7%. The ubiquitinated residues identified are highlighted in blue. d, Spectrum showing FolSvp2 ubiquitination at K215. The representative y2 ion represents the assignment of K215 ubiquitination.

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