Fig. 1: Interploidy and interspecies hybrid seeds share similar phenotypes and deregulated genes.
From: Dosage-sensitive maternal siRNAs determine hybridization success in Capsella
a, Cleared hybrid Capsella seeds at 4 to 6 DAP. The corresponding mature seeds are shown on the right side of each panel. Scale bars, 100 µm and 1 mm for cleared and mature seeds, respectively. The colour code reflects effective ploidy as indicated in the figure. b, Mature seed phenotypes of crosses Cr × Cbp and Cbp × Cr. Scale bars, 1 mm. c, Mature seed phenotypes of crosses Co × Cr and 4xCo × Cr. Scale bars, 1 mm. d, Germination data for the crosses shown in c. Shown is the fraction of germinating seeds from each cross, with each filled circle representing one biological replicate; there are three biological replicates per genotype. The numbers at the top represent total seed numbers. The asterisks represent statistical significance as calculated by one-way analysis of variance with post-hoc Tukey’s honestly significant difference test (***P < 0.001). e, Upset plot showing the overlap of upregulated genes between interploidy (Cr × 4xCr) and interspecies crosses of different Capsella species. f, Heat map and dendrogram show clustering of samples based on log2 fold changes (compared with the corresponding maternal parent) of genes upregulated in the analysed hybrids.
