Fig. 2: Interspecies hybridization causes chromatin condensation defects in endosperm nuclei.
From: Dosage-sensitive maternal siRNAs determine hybridization success in Capsella
a, Percentage of genes in pericentromeric and non-pericentromeric regions that are upregulated in different incompatible Capsella hybrids. The numbers above the bars represent numbers of upregulated genes in pericentromeric and non-pericentromeric regions of a respective genotype. The asterisks represent statistical significance as calculated by two-sided chi-squared tests; P values were adjusted for multiple comparisons with Benjamini and Hochberg correction (***P < 0.001; **P < 0.01; *P < 0.05). b, DAPI-stained chromocenters from endosperm nuclei at 4 DAP of different Capsella genotypes. Scale bars, 5 μm. c, Quantification of chromatin condensation given as mean circularity of chromocenters (Methods). The numbers below the boxes indicate the number of analysed nuclei. The asterisks represent statistical significance as calculated by two-sided Wilcoxon tests; P values were adjusted for multiple comparisons with Benjamini and Hochberg correction (***P < 0.001; **P < 0.01; *P < 0.05). NS, not significant. d, Metagene plots showing DNA methylation levels of TEs in the endosperm of Cg × Cg, 4xCr × Cg and 4xCr × 4xCr 6 DAP seeds. e, Box plots showing methylation levels of TEs in the endosperm of 6 DAP seeds of the indicated genotypes. The asterisks indicate statistically significant differences as calculated by two-sided Wilcoxon tests; P values were adjusted for multiple comparisons with Benjamini and Hochberg correction (***P < 0.001; **P < 0.01). f, Metagene plots showing DNA methylation levels of TEs in the endosperm of Cg × Cr 6 DAP compared with published data for Cr × Cr, Cg × Cg and Cr × Cg (ref. 9). g, Box plots showing methylation levels of TEs in the endosperm of 6 DAP seeds of the indicated genotypes. The asterisks indicate statistically significant differences as calculated by two-sided Wilcoxon tests; P values were adjusted for multiple comparisons with Benjamini and Hochberg correction (***P < 0.001). In c,e,g, the horizontal lines show the median values, the box edges show the interquartile range and the whiskers show the full range excluding outliers.
