Fig. 3: Maternal loss of NRPD1 mimics paternal-excess hybrid phenotypes.
From: Dosage-sensitive maternal siRNAs determine hybridization success in Capsella
a, DAPI-stained chromocenters from endosperm nuclei from Cr × Cr, Cr × nrpd1, nrpd1 × Cr and nrpd1 × nrpd1. Scale bars, 5 μm. b, Quantification of chromatin condensation given as mean circularity of chromocenters (Methods). The numbers below the boxes indicate the number of analysed nuclei. The asterisks represent statistical significance as calculated by two-sided Wilcoxon tests; P values were adjusted for multiple comparisons with Benjamini and Hochberg correction (***P < 0.001). c, Metagene plots showing DNA methylation levels of TEs in the endosperm of Cr × Cr, nrpd1 × nrpd1, Cr × nrpd1 and nrpd1 × Cr 6 DAP seeds. d, Box plots showing methylation levels of TEs in the endosperm of 6 DAP seeds of the indicated genotypes. The asterisks indicate statistically significant differences as calculated by two-sided Wilcoxon tests; P values were adjusted for multiple comparisons with Benjamini and Hochberg correction (***P < 0.001; **P < 0.01). In b and d, the horizontal lines show the median values, the box edges show the interquartile range and the whiskers show the full range excluding outliers. e, Upset plot showing the overlap of upregulated genes among Cr × 4xCr, nrpd1 × nrpd1, nrpd1 × Cr and Cr × Cg derived seeds. Genes were considered as upregulated on the basis of log2(fold change) > 1 and Padj < 0.05 compared with Cr × Cr.
