Extended Data Fig. 9: mRNA Ψ modification in various tissues of Arabidopsis and rice. | Nature Plants

Extended Data Fig. 9: mRNA Ψ modification in various tissues of Arabidopsis and rice.

From: Quantitative RNA pseudouridine maps reveal multilayered translation control through plant rRNA, tRNA and mRNA pseudouridylation

Extended Data Fig. 9

a, BID-seq revealed a large number of mRNA Ψ sites in ten rice tissues. b, Diverse average Ψ fractions on mRNA across different tissues in rice, including plumule (n = 1,468), radicle (n = 1,851), seedling 8DAY (n = 1,153), seedling 2 W (n = 1,781), straw heading (n = 1,710), flag leaf heading (n = 1,631), panicle (n = 1,604), flag leaf 10DAA (n = 1,699), embryo (n = 1,844), and endosperm (n = 1,128) shown in boxplot. The red dots mark the mean, the lines show the median, the boxes represent the interquartile range (IQR), and the whiskers extend to 1.5× of the IQR. c, Boxplot showing fractions of tissue common (n = 123) and unique mRNA Ψ sites in Arabidopsis, including seedling (n = 478), shoot (n = 400), root (n = 1,160), rossta leaf (n = 385), cauline leaf (n = 315), stem (n = 416), flower (n = 706), silique (n = 370), and seed (n = 712). d, Boxplot showing fractions of tissue common (n = 55) and unique mRNA Ψ sites in rice, including plumule (n = 399), radicle (n = 1,042), seedling 8DAY (n = 200), seedling 2 W (n = 552), straw heading (n = 408), flag leaf heading (n = 509), panicle (n = 440), flag leaf 10DAA (n = 482), embryo (n = 728), and endosperm (n = 401). The lines show the median, the boxes represent the interquartile range (IQR), and the whiskers extend to 1.5× of the IQR. 8DAY represents 8 days and 2 W represents 2 weeks. 10DAA represents 10 days after anthesis.

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