Extended Data Fig. 8: Vitamin B6 homeostasis plays an important role in plant defense.
(a) Typical morphological phenotype of Arabidopsis plants treated with water or 1 mM vitamers (PN/PL/PM) prior to inoculation with Pst-avrRpt2 (106 CFU/ml). The plants were photographed 12 h post-inoculation. Arrows indicates pathogen inoculated leaves. (b) H2O2 levels in plants treated with water or 1 mM vitamers (PN/PL/PM) prior to inoculation with Pst-avrRpt2 (106 CFU/ml). Scale bar represent 0.5 cm. (c) Electrolyte leakage in Arabidopsis plants treated with water or 1 mM vitamers (PN/PL/PM) prior to inoculation with Pst-avrRpt2 (106 CFU/ml). Error bars represent SD (n = 5). (d) Plate assay showing growth of Pst-avrRpt2 on Kings B medium in the presence of 20 µM H2O2 or 1 mM vitamers (PN/PL/PM). (e-g) Relative levels of SA (left panel) and SAG (right panel) (e), Pip (f), NHP (left panel) and NHP-glucoside (right panel) (g) in Arabidopsis plants treated with water or 1 mM vitamers (PN/PL/PM) prior to inoculation with Pst-avrRpt2 (106 CFU/ml). (h) Growth of Pst-avrRpt2 on Arabidopsis plants treated with water or 1 mM vitamers (PN/PL/PM) prior to inoculation. The error bars represent SD (n = 3 or 4 biological replicates). Asterisks denote a significant difference (multiple unpaired t test, **P < 0.0021; ****P < 0.0001). The experiments described here were repeated at least twice with similar results.
