Extended Data Fig. 3: SKIP is highly disordered and possesses liquid-like properties in vivo.
a, Prediction of disorder regions of SKIP by PONDR (VLXT). The scores are assigned between 0 and 1, and a score above 0.5 indicates disorder. b, Structure of SKIP protein predicted by AlphaFold. c,d, Cold-induced SKIP condensates are sensitive to 1,6-hexanediol. Representative images in the stele cells in the root elongation zone of cold-treated Arabidopsis with or without 10% 1,6-hexanediol for 90 s (c), and quantification of cells with condensates (d) are shown. In (d), data are means ± s.d. from three biologically independent experiments; statistical significance was determined using a two-tailed t-test. e,f, Time course of FRAP of SKIP nuclear condensates in the nuclei of N. benthamiana leaf cells under normal conditions (e), and in the nuclei of skip-1 proSKIP::SKIP-GFP root cells under cold conditions (f). Time 0 indicates the time of the photobleaching pulse. Data are means ± s.d. (n = 7 independent experiments), and representative images are shown. (g) Quantification of cells with condensates in Fig. 3a. Data are means ± s.d. (n = 3 root elongation zones analyzed in three independent experiments; ~30 nuclei in each root were examined). ND, not detected.
