Extended Data Fig. 4: In vitro phase separation and in vivo protein levels of SKIP with or without cold treatment.
a,b, In vitro phase separation of recombinant His-GFP-SKIP and His-SKIP incubated at 22 °C for 10 min. Representative images (a), and coomassie blue staining of indicated proteins (b) are shown. His-GFP serves as a control. c, In vitro phase separation of recombinant His-GFP-SKIP incubated at 22 °C for 10 min at different concentrations. d, In vitro phase separation of recombinant His-GFP-SKIP incubated at 22 °C and 4 °C for 1 h. His-GFP serves as a control. e, Detection of the SKIP protein level in two-week-old Col-0 and skip-1 using anti-SKIP antibody. f, Detection of the SKIP protein level in two-week-old Col-0 with or without 4 °C treatment. Nuclear proteins were extracted, and H3 was used as a loading control.
