Extended Data Fig. 3: H3K36me3 analysis of the CRISPR-Cas9 based sdg8-SET-KO mutant.
From: H3K36 methylation stamps transcription resistive to preserve development in plants
a, Schematic representation of the SGD8 gene. The sdg8 mutant (efs-3) used in this study was indicated. The red lines refer to the primers for RT-qPCR in the Extended Data Fig. 3b. b, The RT-qPCR to detect the wild-type SDG8 and truncated SDG8 mRNA levels in Col-0 and sdg8-SET-KO, respectively. The values are normalized to the GAPDH gene. Mean +/- SD with three replicates. Statistical significance was determined using an unpaired t-test with a two-tailed p-value. ns, no significance. c, Alignment of the predicted SDG8 SET domain of Col-0 and sdg8-SET-KO based on the Sanger sequencing of RT-PCR products in the Extended Data Fig. 3d. d, The cDNA sequence of sdg8-SET-KO on SET-domain region. e, Metaplot showing the 4 clustered genes using H3K36me3 ChIP-seq data of Col-0 and sdg8-SET-KO. f, Venn diagram illustrating the overlap between the detailed classification of the sdg8-SET-KO H3K36me3 data and sdg8 (efs-3) data. g. Snapshots from Integrative Genomics Viewer showing H3K36me3 signals of Col-0 and sdg8-SET-KO.
