Extended Data Fig. 4: Diverse functions of SDG8 in H3K36me1/2/3 abundance on coding region.
From: H3K36 methylation stamps transcription resistive to preserve development in plants
a, ChIP-seq of H3K36me1, H3K36me2 and H3K36me3 were analyzed by PCA. Each experiment was performed with two replicates. b, Snapshots of a representative region of C1-C4 genes showing indicated chromatin features. c, Left, heatmaps of Col-0 H3K36me1 signal, Col-0 H3K36me2 signal and sdg8 H3K36me2 signal around Col-0 H3K36me1 peak summits. Right, heatmaps of Col-0 H3K36me2 signal, Col-0 H3K36me3 signal and sdg8 H3K36me3 signal around Col-0 H3K36me2 peak summits. d, Left, heatmaps of sdg8 H3K36me1 signal, Col-0 H3K36me2 signal and sdg8 H3K36me2 signal around sdg8 H3K36me1 peaks summits. Right, heatmaps of sdg8 H3K36me2 signal, Col-0 H3K36me3 signal and sdg8 H3K36me3 signal around sdg8 H3K36me3 peak summits. e, Metaplot of H3K36me1/2/3 enrichment on C1-C4 genes in Col-0 and sdg8. f, Working model of H3K36me methyltransferases functions in H3K36me1/2/3 deposition on coding region. On C1 genes, other H3K36 methyltransferases, rather than SDG8, are responsible for the deposition of H3K36me1. The deposition of H3K36me2 on C1 could be catalyzed by all H3K36 methyltransferases while the H3K36me3 on C1 genes was mainly catalyzed by SDG8. On C2 genes, SDG8 and other H3K36 methyltransferases could jointly catalyze all types of H3K36me. On C3 genes, SDG8 could inhibit other H3K36 methyltransferases from catalyzing the over-deposition of H3K36me1, H3K36me2 and H3K36me3. On C4 genes, the low levels of H3K36me1 could be catalyzed by all H3K36 methyltransferases, and the H3K36me2 deposition is mainly catalyzed by SDG8, while very low to no levels of H3K36me3 could be detected on these genes.
