Extended Data Fig. 5: The increased RNA stability for C3 genes.
From: H3K36 methylation stamps transcription resistive to preserve development in plants
a, Boxplots showing the mRNA levels of Col-0 and sdg8 in C1-C4. Normalized reads are shown as log2(count +1). Sample numbers: nC1 = 910, nC2 = 1198, nC3 = 10030, nC4 = 9465. Average values from two biological replicates are shown. Average values from two biological replicates are shown. Statistical significance was determined using an unpaired t-test with a two-tailed p-value. ns, no significance. *, p < 0.05. ****, p < 0.0001. The p values for C1, C2, C3, and C1-C3 are: 2.6E-06, 0.0161, 0.4922, 2.1E-81. Boxplots show the median, 25th-75th percentiles, and minima and maxima (whiskers). The crosses represent the mean values. b, The ratio of m6A modified mRNA in total mRNA from each clustered genes. c, Total RNA abundance of 5-day seedlings on C1-C4 genes. RNA-seq data was obtained from ref. 24. Sample numbers: nC1 = 1670, nC2 = 1236, nC3 = 10710, nC4 = 12878. Average values from two biological replicates are shown. Boxplots show the median, 25th-75th percentiles, and minima and maxima (whiskers). d, Metaplot of H3K36me3 levels on C1-C4 genes using 5-day seedlings. e, Metaplot of Ser2P NET-seq levels on C1-C4 genes using 5-day Col-0 seedlings. f, Snapshots from Integrative Genomics Viewer showing H3K36me3 and NET-seq signals of 5-day seedlings on selected genes. Two replicates are shown.
