Fig. 2: Identification of GC types from the abaxial and adaxial surfaces, and analysis of DEGs in Arabidopsis.
a, A flowchart of scRNA-seq of GCPs in Arabidopsis. b, A visualization of the distribution and numbers of protoplasts for two samples (WT1 and WT2) using t-SNE. c, Isolation of two cell clusters of GCPs for two samples (WT1 and WT2) using t-SNE. Each coloured dot represents individual cell types, namely GC1 and GC2. d, PCA of the scRNA-seq and RNA-seq for the GCPs. Among them, WT1-ab, WT2-ab, WT1-ad and WT2-ad represent the mean results of GC RNA-seq obtained through the tape–peel method in both WT plants. WT1-GC1, WT2-GC1, WT1-GC2 and WT2-GC2 represent the mean results of GCPs scRNA-seq in both WT plants. Percentages represent variance captured by principal components 1 and 2 in each analysis. e, A heat map showing the DEGs in the GCPs from the adaxial and abaxial surfaces of two WT plants by RNA-seq (n = 3). Data were Z-score normalized within a given parameter, and organized by hierarchical clustering. Biological processes are listed, such as response to high light intensity, RNA modification, response to heat, oxidation–reduction processes, transmembrane transport, stomatal movement and photosynthesis. f, qPCR analysis of K+in channels genes in GCPs of the abaxial and adaxial epidermis of WT1 and WT2. The columns and error bars in f represent the mean ± s.d. (n = 6). The letters indicate groups with statistically significant differences (P < 0.05). Data were analysed through one-way ANOVA and Fisher’s multiple comparison with two-tailed tests.
