Extended Data Fig. 6: Similar γH2Ax foci numbers in scep3 when compared with the WT and absence of HEI10 immunofluorescence signals in hei10-2. | Nature Plants

Extended Data Fig. 6: Similar γH2Ax foci numbers in scep3 when compared with the WT and absence of HEI10 immunofluorescence signals in hei10-2.

From: The synaptonemal complex central element SCEP3 interlinks synapsis initiation and crossover formation in Arabidopsis thaliana

Extended Data Fig. 6

a, Immunolocalization of ASY1 and γH2Ax in WT and scep3-2 meiocytes. γH2Ax signal channel is depicted on the right (gray). Scale bar, 10 μm. b, Quantification of γH2Ax foci numbers in Col-0 (187 ± 51 nm, n = 31) and scep3-2 (177 ± 31 nm, n = 30) meiocytes (late leptotene/early zygotene). Data are presented as mean ± standard deviation (s.d.). No significant difference was found between Col-0 and scep3-2 (P = 0.36, two-sided Student’s t-test). N.S., not significant. c, Immunolocalization of HEI10 and ASY1 in hei10-2. The experiment was repeated two times with similar results.

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