Extended Data Fig. 9: Isolation of scep3-4 in Ler-0. | Nature Plants

Extended Data Fig. 9: Isolation of scep3-4 in Ler-0.

From: The synaptonemal complex central element SCEP3 interlinks synapsis initiation and crossover formation in Arabidopsis thaliana

Extended Data Fig. 9

a, Details on the CRISPR/Cas9-mediated allele scep3-4: 2 bp deletion within exon five resulting in a predicted truncated protein of 182 AA. PAM sequence underlined and deleted bases as red dashes. Below: alignment of SCEP3 wildtype and truncated SCEP3-4 protein. b, Seed setting in siliques of Ler-0 and scep3-4. Seed abortion indicated by asterisks. c, Male meiotic chromosome spreads and d, quantification of univalent frequency in Ler-0 and scep3-4.

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