Fig. 7: CO interference and heterochiasmy vanish in scep3.
a, Number of COs (the data are presented as mean ± s.e.m.) detected by sequencing of recombinant offspring of WT males (4.80 ± 0.17), WT females (3.00 ± 0.11), scep3-2 males (6.00 ± 0.24) and scep3-2 females (6.15 ± 0.23). The sample sizes used for analysis are indicated in parentheses. Significant differences were detected between WT males and females (P < 1 × 10−7), but not between scep3-2 males and females (P = 0.942) (nested ANOVA followed by Tukey’s honestly significant difference test, a two-sided test with adjustment for multiple comparisons). Compared with the WT, in scep3-2 CO numbers significantly increased in both males (P = 5.09 × 10−4) and females (P < 1 × 10−7). b, CO distribution along chromosome 2 in male and female WT, zyp1 (ref. 16), scep1-1 (ref. 18) and scep3-2. Centromere and pericentromere regions are indicated in grey and blue, respectively. CO data are presented within 1-Mb windows. Significant differences (based on χ2 tests) between the WT and scep3-2 (green dots), scep3-2 and zyp1 (red dots) and scep3-2 and scep1-1 (orange dots) are indicated. c, Distribution of inter-CO distances (only chromosomes with exactly two COs are included for analysis) in male and female WT and scep3-2. Calculated random distributions are shown in grey. The statistical significance is indicated in parentheses (nested ANOVA followed by Tukey’s honestly significant difference test, a two-sided test with adjustment for multiple comparisons). d, The coefficient of coincidence (CoC) was calculated for inter-interval distances ranging from 1 Mb to 15 Mb for each chromosome, and a LOESS curve was fitted (coloured lines). e, Immunolocalization of ASY1 and HEI10 in WT and scep3-1 female meiocytes. DAPI-stained DNA is shown in blue. Scale bars, 10 μm.
