Extended Data Fig. 3: Functional validation of PSS1 reporter lines.

(a) Siliques of Wt, pss1-3, PSS1:GFP (in pss1-3) line1, and PSS1:GFP (in pss1-3) line2 plants. Bar: 1 cm. (b) Pollen viability of Wt, pss1-3, PSS1:GFP (in pss1-3) line1, and PSS1:GFP (in pss1-3) line2 plants. Asterisks indicate significant difference (one-way ANOVA followed by Tukey’s multiple comparaison test, **** indicate p values < 0.0001, ns= non significant). Magenta lines show mean \(\pm \,\)SD. (c) Expression pattern of PSS1:GFP in male meiosis of pss1-3 mutants at early, mid, and late prophase. Bars: 10 µm.