Extended Data Fig. 7: Localization dependency between SINE3, PSS1, and Cter-SUN proteins – Immunocytology. | Nature Plants

Extended Data Fig. 7: Localization dependency between SINE3, PSS1, and Cter-SUN proteins – Immunocytology.

From: Identification of the cytoplasmic motor–LINC complex involved in rapid chromosome movements during meiotic prophase in Arabidopsis thaliana

Extended Data Fig. 7

Co-immunostaining of REC8 (magenta), with Cter-SUNs or PSS1 or SINE3:interGFP on 3D-preserved male meiocytes from wild-type or mutants. For each cell from Fig. 4d (main text), the maximum intensity projection of each signal is shown. Bars: 2 µm, except for pss1 meiocyte: 3 µm. For the 3D movie stacks, see the following videos:. Movie11_4D_Wt: Wt with anti REC8, and PSS1. Movie12_4D_Wt: Wt with anti REC8, and Cter-SUNs. Movie13_4D_sine3: sine3-1 with anti REC8, and PSS1. Movie14_4D_sine3: sine3-1 with anti REC8, and Cter-SUNs. Movie15_4D_pss1_SINE3interGFP: pss1-1_SINE3:interGFP with anti REC8, GFP, and Cter-SUNs. Movie16_4D_sun1sun2_SINE3interGFP: sun1sun2_SINE3:interGFP with anti REC8, GFP, and PSS1.

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