Extended Data Fig. 9: SINE3 and PSS1 are enriched at telomere anchorage sites on the NE and are required for telomere attachment to the NE and bouquet formation.

(a) Maximum intensity projections of each channel for the cells shown in Fig. 5a (main text), Bars: 2 µm. Corresponding 3D movie stacks are: Movie17_5A_Wt_SINE3interGFP and Movie18_5A_Wt_SINE3interGFP. (b) Maximum intensity projections of each channel for the cells shown in Fig. 5b (main text), Bars: 3 µm. Corresponding 3D movie stacks are: Movie19_5B_Wt, Movie20_5B_sine3, and Movie21_5B_pss1. (c) Example of a mutant meiocyte where telomere cluster is observed in the nucleoplasm. Bars: 3 µm. Corresponding 3D movie stacks are Movie22_sine3 and Movie23_pss1. For B and C, and on 3D movie stacks, nucleolus and NE segmentations are shown, with the telomere locations (coloured spheres). The colour code is based on the proximity to the nuclear periphery (the telomeres at the periphery are the pinker).