Extended Data Fig. 10: The cytoskeletal motor-LINC complex chain (PSS1-LINC) is required for class I CO distribution.

(a) Representative example of late diplotene cells on which the MLH1 foci quantification has been performed (data shown in Fig. 7b, main text). Bars: 3 µm. (b) HEI10 immunostaining in male meiocytes of Wt, sine3-1, pss1-3, sine3-1 pss1-3, and sun1 sun2 mutant plants at late pachytene or late pachytene-like stages. Bars: 10 µm. The red rectangles highlight the chromosome regions with closely localized HEI10 foci. The corresponding quantification of the number of HEI10 foci is shown below. Error bars indicate the mean ± SD and asterisks indicate significant difference (Tukey’s multiple comparison test, P < 0.01).