Extended Data Fig. 1: Phenotypes and Epistatic Analyses of the sine3 Mutants.
(a) Generation and mutation identification of CRISPR-Cas9 sine3 and sine4 mutants. (b) Vegetative growth of Wt, sine3-1, sine3-4, sine3-5, sine4-1, sine4-2, sine3-1 sine4-1, and sine3-1 sine4-2 plants. Bar: 10 cm. (c) Seed viability quantification. Horizontal magenta lines indicate mean \(\pm \,\)SD. Horizontal black lines indicate the results of an ordinary one-way ANOVA, followed by Tukey’s multiple comparison test (comparing all mean values), ns= non significant, **** indicates p values < 0.0001. (d-f) Pollen viability after Alexander staining in single and multiple mutants. Pollen grains from at least five different plants of each genotype were analyzed. Horizontal magenta lines indicate mean \(\pm \,\)SD. Asterisks indicate differences that were found significant after ordinary one-way ANOVA, followed by Tukey’s multiple comparison test (comparing all mean values) (d, e), or unpaired t test (f). ns= non significant, **** indicates p values < 0.0001. (g) Box plot that shows the quantification of the minimum chiasma number at metaphase/anaphase transition. The boxes extend from the 25th to 75th percentiles, whiskers extend from minimum to maximum values. Asterisks indicate the results of an ordinary one-way ANOVA, followed by Dunnett’s multiple comparison test (comparing each mean value to the Wt mean values). ns= non significant, **** indicates p values < 0.0001.
