Extended Data Fig. 5: GHR depends on RIIIDa to control DCL1 cleavage sites.
From: Key RNA elements influencing DCL1 cleavage in plant microRNA biogenesis
(a) Model of the structure and the pre-miRNA substrate containing GHR at DCL1 cleavage site. (b) Schematic diagram of DCL1 and its mutants used in this study. The numbers indicate the amino acid positions, the white boxes indicate deletion area. (c) The purity of proteins was assessed by SDS-PAGE. The purification of DCL1 variants was repeated once for each mutation. (d) A schematic representation illustrates the DCL1-pre-miR-166f complex during the dicing state (PDB: 7ELE). Residue E1507 interacts with the C-G pair situated within the minor groove by forming a hydrogen bond with the -NH2 group of the guanine base.
