Extended Data Fig. 5: ROS1 regulates TE expression via its demethylase activity.
From: Plants repress ROS1 expression to attenuate heat-induced transposon burst
a-c, IGV snapshots showing DNA methylation levels at ONSEN, ROMANIAT5, AtMU1, AtMU2 and AtGP10 loci in Col-0 (22 °C), Col-0 (37 °C), ros1-4 (37 °C) and SP:ROS1-GFP (37 °C) plants. TE promoter regions are highlighted by red boxes. d, Bisulfite sequencing data showing CHH methylation levels in the regions spanning upstream, LTR, and partial ORF of ONSEN2 (AT3TE92525) in Col-0 (22 °C), Col-0 (37 °C), and SP:ROS1-GFP (37 °C) plants. At least 20 clones were sequenced for each sample. Each circle represents one biological replicate (n = 2 biological replicates). LTR, long terminal repeat; ORF, open reading frame; Red line represents the genomic location of BS-PCR amplification products. e, Relative quantification of ROMANIAT5, AtMU1, and TSI expression levels in Col-0, SP:ROS1-Flag, and SP:ROS1C1045S-Flag transgenic lines under control and heat-stress conditions. Data represent mean ± s.d., n = 3 biological replicates. Two-way ANOVA with Bonferroni’s multiple comparisons test.
