Extended Data Fig. 8: Colocalization analysis of GFP-VSR1 with endosomal markers after infection with Pto DC3000 avrRpm1.
(a-c) Colocalization analysis of GFP-VSR1 with the MVB/PVC marker mCherry-Rha1 (a), Golgi marker mCherry-SYP32 (b), and TGN marker VHA-a1-RFP (c). Confocal images are representative of mesophyll cells in leaves of 4-week-old plants before (0) and after (3 h) inoculation of the Pto DC3000 avrRpm1. (d) Colocalization analysis of mCherry-Rha1 with the autophagosome marker eYFP-ATG8e in leaves of 4-week-old plants before (0) and after (3 h) inoculation of the Pto DC3000 avrRpm1. Separated images of each channel in the white outlined area are shown on the right side (from top to bottom: GFP/eYFP, RFP/mCherry, and merged). Arrows indicate the colocalized fluorescence. The colocalization percentage is included in the bottom. Colocalization was quantified from 10 individual leaves of three independent experiments. Data are presented as means ± SD. n, total numbers of analyzed punctae. ****P < 0.0001; n.s., P > 0.05 in two-tailed unpaired Student’s t-test. Scale bars, 10 μm.
