Extended Data Fig. 5: Subcellular location of PyCHD, PbKAT and PbTE in Nicotiana benthamiana leaves. | Nature Plants

Extended Data Fig. 5: Subcellular location of PyCHD, PbKAT and PbTE in Nicotiana benthamiana leaves.

From: Naturally impaired side-chain shortening of aromatic 3-ketoacyl-CoAs reveals the biosynthetic pathway of plant acetophenones

Extended Data Fig. 5

N-terminal GFP- and mCherry-tagged constructs of PyCHD, PbKAT, PbTE, and peroxisomal marker px-rk were transiently co-expressed. Fluorescence signals were visualized using confocal microscopy, with GFP (green), mCherry (magenta), and chlorophyll autofluorescence (blue) shown in separate panels. The overlay of GFP, mCherry and chlorophyll autofluorescence signals in the boxed-areas are enlarged to show details in Merged panel.

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