Extended Data Fig. 1: Generation of the R2-BT line. | Nature Plants

Extended Data Fig. 1: Generation of the R2-BT line.

From: Enhancing local meiotic crossovers in Arabidopsis and maize through juxtaposition of heterozygous and homozygous regions

Extended Data Fig. 1

a, Schematic illustrating the creation of R2-BT through single recombinant crosses between Col-BT/Ler (R) carrying only one reporter gene. Successive backcrosses aim to clean up the Ler background. b, The genetic structure of the BT interval for recombinants #112 (RRG-/G-, left) and #130 (R−R/−R, right) used to create R2-BT, determined by targeted sequencing of the BT region. The plots show the percentage of Col alleles (blue) and Ler alleles (red) across the entire BT region. The dashed rectangles indicate the location of Col/Ler crossover breakpoints. c, The genetic structure of R2-BT determined through whole-genome sequencing based on short reads. Polymorphisms indicating the presence of Ler-derived fragments are marked in gray. The BT region on chromosome 3 is indicated by a red line.

Back to article page