Fig. 3: The THFS/MTHFD1-dependent pathway supplies C1 units from formate for homocysteine-to-methionine recycling, DNA methylation and thymidylate synthesis.
From: Photorespiration is linked to DNA methylation by formate as a one-carbon source
a, Label enrichment in methionine after [13C]formate supplementation at the beginning (left) or end (right) of the photoperiod. The grey shading depicts dark periods. The data are presented as mean values ± s.d. (n = 3). See Supplementary Table 16 for the P values. b, Label enrichment in serine after [2-13C]glycine supplementation at the beginning of the photoperiod. Dual labelling (M + 2) results from combined glycine cleavage and glycine-to-serine conversion in mitochondria. The grey shading depicts dark periods. The data are presented as mean values ± s.d. (n = 3). See Supplementary Table 17 for the P values. c, Label enrichment in targeted nucleobases after [13C]formate supplementation. Guanine was not quantified due to low recovery (Methods). The data are presented as mean values ± s.d. (n = 3). Lowercase letters represent significant differences between genotypes for each nucleobase (P < 0.05, one-way analysis of variance followed by post-hoc Tukey test). See Supplementary Table 18 for the P values. 5mC, 5-methylcytosine.
