Fig. 2: The gut-brain axis mediates the development of mammary sensory nerve.

The blank control group (4-week-old pubertal mice) was not subjected to any treatment, the negative control group (4/6-week-old pubertal mice) was exerted with SS, and the experimental group (4/6-week-old pubertal mice) was exerted with Vago (n = 6); (a) The protein expression of Tuj1 and BDNF in MG were detected by Western blotting (n = 3); (b, c) Relative protein abundance of Tuj1 and BDNF (compared to β-actin) in Fig. 2a; (d) ELISA kit for measuring BDNF in serum; (e) MG frozen sections were stained with Tuj1 (red) and DAPI (blue) to visualize sensory nerve fibers (n = 5), scale bar: 100 μm; (f) Quantification of mammary sensory nerve fiber density. The mean ± SEM was used for all data presentations. Compared to the SS group, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.