Fig. 7: Initial microbial inoculation ratio (by dilutions) affects metabolism fluctuations and community assembly within a simulated fermentation ecosystem.
From: Controlling metabolic stability of food microbiome for stable indigenous liquor fermentation
a Schematic of the experimental design under different initial inoculation conditions. b Changes in metabolites during initial and medium fermentation phases reveal different patterns of metabolic expression levels. The abscissa is each comparative sample group, and the ordinate is the expression level of metabolites in groups. Each gray line represents a metabolite, and the blue line represents the average expression of all metabolites in the sub-cluster. Different metabolites detected by UPLC-MS/MS are grouped into sub-clusters via H cluster. c Bray-Curtis distance between the four groups to the control group based on volatile metabolic profiles during fermentation. SD represents the standard deviation of Bray-Curtis distances to reflect metabolic stability. d Community assembly (blue) patterns and metabolic stability (red) at four dilution gradients during the first 15 days of fermentation. The intersection of the fitted lines suggests the ideal microbial inoculation ratio.
