Fig. 4: Effect of Ba 6 on dsRNA stability and host RNAi efficiency.
From: Bacillus secretes nucleases to degrade dsRNA, thereby reducing host’s susceptibility to RNAi
a dsRNA degradation capability in Ba 6-colonized cotton bollworms. LB + dsEGFP, LB + dsEGFP-Cy3, Ba 6 + dsEGFP-Cy3: Cotton bollworms treated with LB and dsEGFP, LB and dsEGFP-Cy3, and Ba 6 and dsEGFP-Cy3, respectively. BF Bright field image; 540 nm: Excitation wavelength; scale bar: 0.5 cm. b, c Relative expression of target genes CarE and BtR after dsCarE and dsBtR injection in Ba 6-colonized cotton bollworms. d Expression levels of Ago-2 and Dicer in different treatment groups (CK, LB, Ba 6). e Changes in Ago-2 and Dicer expression in Ba 6-colonized cotton bollworms and LB medium-treated controls after dsRNA injection. Effects on target gene inhibition efficiency (f, h, j, l) and cotton bollworm survival rate (g, i, k, m) following feeding with Ba 6 suspension or supernatant, and subsequent feeding with dsCarE or dsTm-1-containing diet. Data analysis in (b–e, f, h, j, l) was performed using Student’s t-test: ***P < 0.001; **P < 0.01; *P < 0.05. Survival data in (g, i, k, m) were analyzed by Log-rank (Mantel-Cox): ***P < 0.001; **P < 0.01; *P < 0.05.
