Fig. 5: Establishment and validation of the Serratia-Fusobacterium prognostic scoring model for LSCC recurrence.

A Statistical analysis of the amounts of Serratia and Fusobacterium between the NR and RC groups in the LSCC cohort was calculated using the Mann–Whitney test. B The correlation between the amounts of Serratia and Fusobacterium, as determined by quantitative real-time polymerase chain reaction (qPCR) and the abundance as measured by 16S rRNA sequencing in the LSCC cohort. C DFS was determined in patients with LSCC from the LSCC cohort who were stratified by the cut-off value of the amounts of Serratia and Fusobacterium, as assessed by the log-rank test. D Multivariate analyses of the level of Serratia and Fusobacterium for DFS were conducted in the LSCC cohort. The bars represent 95% confidence intervals. E Schematic illustration of the generation of microbial prognostic scores in the Serratia-Fusobacterium prognostic scoring model (SF model). F ROC curve analysis was performed based on the levels of Serratia and Fusobacterium alone, SF model, and the TNM staging system to discriminate LSCC recurrence in the LSCC cohort. G DFS was determined in patients with LSCC from the LSCC cohort who were stratified by the status of the SF model, as assessed by the log-rank test. H ROC curve analysis was performed based on the level of Serratia and Fusobacterium alone, SF model, and the TNM staging system, for discriminating LSCC recurrence in the Multi-center LSCC cohort. I DFS was determined in patients with LSCC from the Multi-center LSCC cohort who were stratified by the status of the SF model, as assessed by the log-rank test.