Fig. 6: Associations of MYC and host AA transport after the colonization of P. succinatutens.

Comparison of colonic glutamine (A), glutamate (B), arginine (C), and serum arginine (D) levels among three groups (n = 6). E PCA plot displays the difference in gene expression profiles among three groups (n = 4). Volcano plot displays the number of DEGs from two comparison groups, including HFD vs. Cont (F) and HFD + Ps vs. HFD (G). Colors with yellow and green dots represent upregulated and downregulated DEGs, respectively. H The overlap genes identified by taking the intersection between upregulated DEGs in HFD vs. Cont and downregulated DEGs in HFD + Ps vs. HFD. I mRNA levels of the overlap genes Lamb3, Elovl3, Serpinbla, Clstn3, and Mmp12 among three groups (n = 4). J KEGG pathway analysis of upregulated DEGs in HFD + Ps group. K The DEGs involved in arginine biosynthesis, including Gls2, Got1, Cps1, Ass1, and Asl, are depicted by the KEGG pathway map. Heatmap displays the mRNA levels of the above genes. L Expression levels of genes involved in arginine synthesis, including Gls2, Cps1, Otc, Ass1, and Asl quantified by qPCR analysis among three groups (n = 6). Western blot images (M) and quantization (N) of gene-encoded proteins FFAR3, MYC, glutamine transporters (SLC1A5 and SLC38A5), arginine synthesis (GLS2, CPS1, and ASL) among three groups (n = 3). Cont: control group, HFD: high-fat diet group, HFD + Ps: mice that received HFD were gavaged with P. succinatutens suspension. Differences among the three groups were assessed using ordinary one-way ANOVA. Data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant difference.