Fig. 1: Study design.
From: High-fat and low-fiber diet elevates the gut resistome: a comparative metagenomic study
The study was conducted in two stages. First, a mouse experiment was performed to investigate the effect of different diets on the microbial community, resistome, mobilome and virulome. In second stage, we retrieved a human dataset containing complete metagenomics data from healthy individuals, along with dietary and demographic information, to determine whether similar dietary effects on the gut resistome observed in mice could be confirmed in humans. The analysis based on dietary habits and BMI was noted as prat I and part II respectively. Due to the minimal number of 5 individuals with high-fat/low-fiber diet and 11 individuals with obese, we ultimately included 5 and 11 as a minimum sample size of corresponding group for part I and part II analysis, respectively. The remaining groups were matched by gender and age in a 1:2 ratio. If the number of available individuals for matching was less than twice that of the high-fiber or obesity groups, we opted for a 1:1 ratio for comparative analysis.
