Fig. 3
A Oncoprint depicting somatic pathogenic/likely pathogenic alterations detected in 95/143 HR + /HER2− advanced breast cancer patients. The upper bar graph illustrates the number of alterations per sample, while the right bar graph shows the frequency of alterations for each gene. B Comparison of mutation frequencies between 1 L (N = 62) and 2 L (N = 25) liquid biopsy samples. Bar plots display the percentage of cases with mutations in key genes, stratified by mutation type: missense mutations (green), multi-hit mutations (yellow), nonsense mutations (blue), and splice-site mutations (red). ESR1, AKT1, and RB1 mutations were numerically more frequent in 2 L samples, while the frequencies of PIK3CA, TP53, and SMAD4 mutations were similar across both groups.
