Fig. 2: Intranasal administration of O-αSyn induced nitrated-αSyn pathology in brain.
From: Circadian disruption promotes the neurotoxicity of oligomeric alpha-synuclein in mice
A Schematic representation of the experimental procedure. A total 26 mice per group were injected with O-αSyn or PBS. At 1 week post injection (w.p.i.), 8 mice were sacrificed (n = 4 for ELISA and n = 4 for innunostaining). At 1 month post injection (m.p.i.), non-motor and motor behaviors were performed, while at 6 m.p.i. and 15 m.p.i., only motor behaviors were performed. 6 of the mice were sacrificed for immunostaining at each time point. B Co-localization of nitrated-αSyn with MAP2, NeuN, Iba1 and GFAP in M1 of the mice at 1 m.p.i. Scale bar = 20 μm.
