Fig. 6: Inhibition of glycolysis decreased the levels of histone lactylation in microglia of PD mice and LPS-stimulated primary microglia.

A Representative images of IBA1 (green) and H3K9la (red) in the SN of Control, MPTP, and 2-DG + MPTP mice. Scale bar, 50 μm. B Quantification of fluorescence intensity of H3K9la in microglia in the SN of Control, MPTP, and 2-DG + MPTP mice. N = 8 per group. C Representative images of IBA1 (green) and H3K9la (red) in the SN of Control, LPS (SN injection) and 2-DG + LPS (SN injection) mice. Scale bar, 50 μm. D Quantification of fluorescence intensity of H3K9la in microglia in the SN of Control, LPS (SN injection) and 2-DG + LPS (SN injection) mice. N = 8 per group. E–G Representative western blotting and quantification of Pan-Kla and H3K9la in the SN of Control, LPS (SN injection), 2-DG and 2-DG + LPS (SN injection) mice. N = 6 per group. H–J Representative western blotting and quantification of Pan-Kla and H3K9la in primary microglia treated with or without sodium oxalate (5 mM) for 30 min, then stimulated with 0.2 μg/mL LPS for 24 h. N = 3 per group. (K) Representative images of IBA1 (green) and H3K9la (red) in primary microglia treated with or without 2-DG (0.5 or 1 mM) for 30 min, then stimulated with 0.2 μg/mL LPS for 24 h. Scale bar, 100 μm. (L) Quantification of fluorescence intensity of H3K9la. N = 3 per group. Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test. Compared to the Control group, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001; compared to the MPTP group or LPS (SN injection) group or LPS group, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001.