Fig. 8: Effects of cuproptosis induction on hub gene expression in A2058 melanoma cells.

A Western blot analysis showed reduced FDX1 protein levels in A2058 melanoma cells treated with 100 or 200 nM elesclomol and CuCl₂ for 24 h, indicating successful induction of cuproptosis. B Quantification of the relative fluorescent intensity of FDX1 normalized to β-actin from Western blot, presented as mean ± SEM (n = 3). C RT-qPCR analysis revealed significant changes in hub gene expression post-cuproptosis induction, including upregulation of cell cycle-related genes (CCNB1, CCNH, CENPE, CENPK, ZWILCH, ZWINT), cytoskeletal organization genes (CORO1C, GSN), and the DNA repair gene (RMI2), as well as downregulation of FOXO3. Upregulation of the PD-associated gene SNCA and the sirtuin gene SIRT2 is also observed, with data presented as mean ± SEM (n = 3).