Fig. 5: dLight responses to electrical stimulation are not altered in VKI brain slices at 3 months.

A i) Graphic depicting stereotaxic injection of AAVs encoding for dLight in dorsolateral STR 4-6 weeks prior to acute slice preparation. Area of dLight expression in coronal slices containing CTX and STR depicted in blue with Stim placed in dorsolateral STR. ii) Visualization of dLight change in fluorescence from baseline (ΔF/F) in response to local electrical stimulation (ΔF/F values of 0 to 1 represented as a gradient from purple to white, respectively). Boundaries of the CC separate CTX and STR in a coronal brain slice. Dashed circle represents ROI of 40 pixels used for dLight analysis. iii) Representative dLight response in dorsolateral striatum corresponding to 2 pulses delivered with 4 s inter-pulse interval. At 3 months iv) Increasing stimulus intensity did not alter size of dLight responses in VKI vs WT STR (2-way ANOVA interaction p = 0.69, genotype p = 0.83). v) Decay of the 1st response peak (P1) is similar between VKI and WT brain slices (Unpaired t-test p = 0.57). vi) PPR at 4 s (P2 Peak/P1 Peak) is similar between VKI and WT brain slices (Unpaired t-test p = 0.21). B i) Representative dLight response in dorsolateral STR corresponding to 4x stimulation with 10 x 10 Hz pulse train and subsequent recovery stimulation. At 3 months ii) dLight responses were not significantly different in size between VKI and WT brain slices (2-way ANOVA interaction p = 0.99, genotype p = 0.85). iii) dLight responses relative to 1st pulse (P1) were not different between VKIs and WTs (2-way ANOVA interaction p = 0.69, genotype p = 0.45). iv) Recovery capacity following 10x10Hz stimulation are lower in VKI brain slices (2-way ANOVA interaction p = 0.81, genotype p = 0.06). v) Decay of the pulse train response is not significantly different in VKI vs WT brain slices (Unpaired t-test p = 0.28).