Fig. 10: A set of aSyn antibodies captures the morphological spectrum of aSyn pathology in synucleinopathy brain tissues.
From: Differential role of C-terminal truncations on alpha-synuclein pathology and Lewy body formation
a WB analyses of the truncation pattern of aSyn in human brain tissue from MSA patients and healthy controls. Cell lysates were analyzed by immunoblotting after sequential extractions of the soluble and insoluble fractions. aSyn species were detected using 1–20, SYN-1, or 134–138 or pS129 antibodies. A double red asterisk indicates the 15 kDa band, the 12 kDa band by a single red asterisk, and the purple arrows indicate the intermediate aSyn-truncated fragments. WB band intensities of total aSyn (15 and 12 kDa) were quantified in the insoluble fraction by densitometry and normalized to actin levels. The level of the 15 kDa aSyn species in the insoluble fraction of the MSA patients is expressed as fold change relative to healthy controls. a.u (arbitrary units). b, c Serial sections from the midbrains of PDD (pars compacta) and SNCA duplication (tegmentum) cases were stained with aSyn antibodies raised specifically against the N-terminal (epitope: 1–20), the NAC (91–99), the C-terminal (epitopes: 110–115 and 134–138) or pS129 (EP1536Y) regions. Scale bars = 50 μm. All the original uncropped and unprocessed WB scans are available in Supplementary Fig. 17.
