Table. 1 Media and reagents used for the differentiation of iPSC cells into dopaminergic neurons
From: Differential role of C-terminal truncations on alpha-synuclein pathology and Lewy body formation
Medium | Product name | Supplier | Catalog no | Working | Time added |
|---|---|---|---|---|---|
KO DMEM KSR | Knockout DMEM | Life Technologies | 10829018 | n/a | 100% DIV 0-4 75% DIV 5-6 50% DIV 7-8 25% DIV 9-10 |
KnockOut Serum Replacement | Life Technologies | 10828010 | n/a | ||
L-Glutamine | Life Technologies | 25030-024 | 2 mM | ||
MEM NEAA | Life Technologies | 11140-050 | 1× | ||
β-mercaptoethanol | Gibco | 21985023 | 10 µM | ||
NNB | Neurobasal Medium | Life Technologies | 21103049 | n/a | 25% DIV 5-6 50% DIV 7-8 75% DIV 9-10 |
N2 Supplement | Life Technologies | 17502048 | 0.5× | ||
B-27® Supplement w/o Vit A | Life Technologies | 12587010 | 0.5× | ||
L-Glutamine | Life Technologies | 25030-024 | 2 mM | ||
NB | Neurobasal Medium | Life Technologies | 21103049 | n/a | DIV11- |
B-27® Supplement w/o Vit A | Life Technologies | 12587010 | 1× | ||
L-Glutamine | Life Technologies | 25030-024 | 2 mM | ||
Growth factors | SB431542 | Bio-techne (Tocris) | 1614 | 10 µM | DIV0-4 |
LDN193189 | Sigma | SML0559-5MG | 100 nM | DIV0-10 | |
Sonic Hedgehog C24II high activity | Bio-techne (Tocris) | 1845-SH-500 | 100 ng/mL | DIV1-6 | |
Purmorphamine | Bio-techne (Tocris) | 4551/10 | 2 µM | DIV1-6 | |
FGF8a | Stratech | 16124-HNAE-SIB | 100 ng/µL | DIV1-6 | |
CHIR99021 | Bio-techne (Tocris) | 4423 | 3 µM | DIV3-12 | |
TGFβ3 | Peprotech | 100-36E | 1 ng/mL | DIV13- | |
DAPT | Abcam | ab120633 | 10 µM | DIV13- | |
db-cAMP | Sigma | D0627-1g | 0.5 mM | DIV13- | |
GDNF | Peprotech | 450-10 | 20 ng/µL | DIV13- | |
BDNF | Peprotech | 450-02 | 20 ng/µL | DIV13- | |
Ascorbic acid | Sigma | A4544-25G | 0.2 mM | DIV13- |
