Fig. 3: Urea cycle activation in PD brains.
From: Urea cycle dysregulation drives metabolic stress and neurodegeneration in Parkinson’s disease
A Urea levels in the substantia nigra and striatum of mice. n = 3 (B) Expressions of key urea cycle enzymes and urea channel proteins in the substantia nigra of mice by Western Blot. n = 3. C Relative expression statistics of urea cycle key enzymes and urea channel proteins in the substantia nigra of mice. n = 3. D Expressions of urea cycle key enzymes and urea channel proteins in the striatum of mice by Western Blot assay. E Western Blot analysis of relative expression statistics of key urea cycle enzymes and urea channel proteins in mouse striatum. F Measurement of ammonia and putrescine level changes in substantia nigra and striatum in the MPTP model. n = 3. G Mice dopaminergic neurons in substantia nigra co-labeled with ODC1 immunofluorescence (short bar = 100 μm, long bar = 10 μm). H Mouse dopaminergic neurons in substantia nigra co-labeled with ARG1 immunofluorescence (short bar = 100 μm, long bar = 10 μm). I Mice striatum dopaminergic neurons co-labeled with ODC1 immunofluorescence (short bar = 500 μm, long bar = 10 μm). J Mouse striate dopaminergic neurons co-labeled with ARG1 immunofluorescence (short bar = 500 μm, long bar = 10 μm). Data are presented as the mean ± SEM. Significant differences were established by Student’s t-test. ***p < 0.001, **p < 0.01, and *p < 0.05.
